Determining if a rabbit has been spayed (female) or neutered (male) can be challenging to do visually, especially if there are no visible surgical scars. However, there are a few signs that may indicate that a female rabbit has been spayed:

1. Absence of Heat Cycles: Female rabbits that have been spayed will no longer go into heat or display signs of reproductive behavior such as nesting, mounting, or aggression towards other rabbits.

2. No Mammary Development: Spaying eliminates the hormonal changes associated with pregnancy and lactation. As a result, spayed female rabbits will not develop mammary glands or produce milk.

3. Decreased Aggression: Female rabbits that have been spayed often exhibit reduced territorial or aggressive behaviors, as the removal of the ovaries reduces hormone-driven aggression.

4. Improved Health: Spaying can have long-term health benefits for female rabbits, reducing the risk of uterine cancer, pyometra (uterine infection), and other reproductive-related illnesses.

However, it's important to note that these signs are not definitive proof of spaying. If you are uncertain about the spay status of a rabbit, it is best to consult with a veterinarian who can perform a physical examination or conduct further diagnostic tests, such as ultrasound, to confirm if the rabbit has been spayed.